RELION 3D classification

For general information on job submission, please see here.

Input particle stack format:

RELION-extracted particle stacks. Click here to learn what this means.

Required input parameters:  

  1. Number of classes: Please provide the number of classes into which particles will be divided
  2. Number of iterations: Provide number of iterations over which to run 3D classification
  3. Particle diameter: Provide particle diameter in Angstroms
  4. Reference map: A single mrc 3D volume is required to start classification

Software citation:

“RELION: Implementation of a Bayesian approach to cryo-EM structure determination.” S.H.W. Scheres J. Struct. Biol. (2012) 180:519-530. Link